TABLE 2.
MICs of various drugs against M. chelonae ATCC 35752, M. chelonae 9917, M. chelonae 9917 expressing mspA (9917/pZS01), and M. chelonae ATCC 35752 expressing mspA (ATCC/pZS01)a
| Drug | MIC (μg ml−1) against:
|
|||
|---|---|---|---|---|
| ATCC 35752 | ATCC/pZS01 | 9917 | 9917/pZS01 | |
| AMP | >500 | >500 | >500 | >500 |
| KAN | 50 | ND | 10 | ND |
| STR | 200 | 250 | 10-20 | 10 |
| VAN | 5 | 5-10 | >500 | 25 |
| CLA | 25-50 | 50 | >250 | 25 |
| AZI | 500 | 500 | 500 | 500 |
| HYG | >500 | ND | >500 | ND |
| ERY | 20-25 | 0.25 | 500 | 1 |
| RIF | 25 | 5 | >500 | 100 |
| EMB | >500 | 25 | >500 | 250 |
| CHL | >500 | 50 | >500 | 50 |
| TOB | 25-50 | ND | 25 | ND |
| GEN | 7.5 | 7.5 | 15 | 20 |
| INH | >400 | >400 | >400 | >400 |
| ETH | >400 | 50 | >400 | 50 |
| PZA | >500 | ND | >500 | ND |
| TET | 50 | 5-10 | >500 | 25 |
| LIN | 25 | 5 | 100 | 1 |
| CIP | 1.5 | 2.5 | 5 | 1 |
| NOR | 5 | ND | 5 | ND |
MICs were determined using the colorimetric resazurin microtiter assay in 7H9-oleic acid-albumin-dextrose-catalase broth at 30°C (16), and results were confirmed by visually scanning for growth. All assays were performed on well-dispersed bacteria and repeated at least three times on independent culture batches. AMP, ampicillin; AZI, azithromycin; CHL, chloramphenicol; CIP, ciprofloxacin; CLA, clarithromycin; EMB, ethambutol; ERY, erythromycin; ETH, ethionamide; GEN, gentamicin; HYG, hygromycin; INH, isoniazid; KAN, kanamycin; LIN, linezolid; NOR, norfloxacin; PZA, pyrazinamide; RIF, rifampin; STR, streptomycin; TET, tetracycline; TOB, tobramycin; VAN, vancomycin; ND, not determined.