TABLE 1.
Strains used in this study
| Strain | Genotype and/or description | Reference or source |
|---|---|---|
| S. avermitilis ATCC 31267 | 18, 27 | |
| S. coelicolor | ||
| M600 | Prototroph, SCP1− SCP2− | 1 |
| B725 | M600 trpRS1::apr; indolmycin sensitive | 20 |
| B730 | B725 trpRS2(L9F), spontaneous mutant selected at 1 μg/ml indolmycin (parent of B737) | 36 |
| B731 | B725, spontaneous mutant selected at 1 μg/ml indolmycin (unknown mutation[s], parent of B734) | This study |
| B732 | B725, spontaneous mutant selected at 1 μg/ml indolmycin (unknown mutation[s], parent of B735) | This study |
| B733 | B725, spontaneous mutant selected at 1 μg/ml indolmycin (unknown mutation[s], parent of B736) | This study |
| B734 | B725 TrpRS2(H48N), spontaneous mutant selected at 10 μg/ml indolmycin | This study |
| B735 | B725 TrpRS2(H48Q), spontaneous mutant selected at 10 μg/ml indolmycin | This study |
| B736 | B725 TrpRS2[G(−13)T], spontaneous mutant selected at 10 μg/ml indolmycin | This study |
| B737 | B725 TrpRS2[C(−17)A/L9F], spontaneous mutant selected at 10 μg/ml indolmycin | This study |
| B738 | B725 pJS310 | This study |
| B739 | B725 pJS311 | This study |
| B740 | B725 pJS312 | This study |
| B741 | B725 pJS313 | This study |
| B742 | B725 pJS314 | This study |
| B743 | B725 pJS315 | This study |
| B744 | B725 pJS316 | This study |
| B745 | B725 pJS317 | This study |
| B746 | B725 pJS318 | This study |
| B747 | B725 pJS319 | This study |
| B748 | B725 pJS320 | This study |
| B749 | B725 pJS321 | This study |
| B750 | B725 pJS322 | This study |
| B751 | B725 pJS323 | This study |
| B752 | B725 pJS324 | This study |
| B753 | B725 pJS325 | This study |
| S. griseus | ||
| NBRC 13350 | 26 | |
| ATCC 12648 | 28 | |
| S. lividans TK24 | ||
| S. venezuelae ATCC 10712 | ||
| E. coli | ||
| DH5α | F− φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK− mK−) phoA supE44 thi-1 gyrA96 relA1 λ− | Invitrogen |
| ET12567 | dam dcm hsdS cat tet | 36 |