TABLE 2.
Plasmids used in this study
| Plasmid | Description | Vector backbone | Reference or source |
|---|---|---|---|
| pUZ8002 | RP4 derivative, OriT, Kanr | 36 | |
| pGemT | pUC-derived, lacZ′, Ampr | Promega | |
| pBluescript KS+ | pUC ori Ampr | Agilent Technologies (Stratagene) | |
| pMS81 | oriT, ΦBT1 attB-int, Hygr | pSET152 | 10 |
| pIJ10257 | oriT, ΦBT1 attB-int, Hygr, ermEp* | pMS81 | 12 |
| pJS310 | ermEp*-SCO3334 ORF (trpRS1) | pIJ10257 | This study |
| pJS311 | ermEp*-SAV4725 ORF | pIJ10257 | This study |
| pJS312 | ermEp*-SGR3809 ORF | pIJ10257 | This study |
| pJS313 | S. coelicolor TrpRS2(WT) locus | pMS81 | This study |
| pJS314 | S. coelicolor TrpRS2[G(−13)T] locus | pMS81 | This study |
| pJS315 | S. coelicolor TrpRS2[G(−13)T/L9F] locus | pMS81 | This study |
| pJS316 | S. coelicolor TrpRS2 [G(−13)T/H48Q] locus | pMS81 | This study |
| pJS317 | S. coelicolor TrpRS2[C(−17)A] locus | pMS81 | This study |
| pJS318 | S. coelicolor TrpRS2[C(−17)A/L9F] locus | pMS81 | This study |
| pJS319 | S. coelicolor TrpRS2[C(−17)A/H48Q] locus | pMS81 | This study |
| pJS320 | ermEp*-S. coelicolor TrpRS2(L9F) ORF | pIJ10257 | This study |
| pJS321 | ermEp*-S. coelicolor TrpRS2(H48Q) ORF | pIJ10257 | This study |
| pJS322 | ermEp*-S. coelicolor TrpRS2(L9F/H48Q) ORF | pIJ10257 | This study |
| pJS323 | ermEp*-S. coelicolor TrpRS2(Q13K) ORF | pIJ10257 | This study |
| pJS324 | ermEp*-S. coelicolor TrpRS2(L9F/Q13K) ORF | pIJ10257 | This study |
| pJS325 | ermEp*-S. coelicolor TrpRS2(H48N) ORF | pIJ10257 | This study |
| pJS326 | SGI trpRS2 full-length cDNA from indolmycin producer | pGemT | This study |
| pJS327 | SGI3809cDNA fragment from indolmycin producer | pGemT | This study |
| pJS328 | S. coelicolor TrpRS2(WT) ORF | pBluescript KS+ | This study |
| pJS329 | S. coelicolor TrpRS2(L9F) ORF | pBluescript KS+ | This study |
| pJS330 | S. coelicolor TrpRS2[G(−13)T] locus | pBluescript KS+ | This study |