TABLE 4.
Comparison of indolmycin resistance genes from Streptomyces species
| Entry | ORFa expressed in B725 (indolmycin-sensitive S. coelicolor) | MICb (μg/ml) |
|---|---|---|
| 1 | S. coelicolor B725 (trpRS1 null strain) | 0.25 |
| 2 | Wild-type S. coelicolor | >150 |
| 3 | S. coelicolor trpRS1 (SCO3334) | 10 |
| 4 | S. avermitilis SAV4725 (trpRS1 homolog) | 5 |
| 5 | S. griseus NBRC 13350 SGR3809 | 15 |
a
All ORFs were introduced into strain B725 under the ermE* promoter for equalized expression in vivo (see Materials and Methods).
b
The MIC is the concentration of indolmycin sufficient to inhibit the growth of each strain after 2 days on Difco nutrient agar (see Materials and Methods).