TABLE 1.

In vitro whole-cell susceptibility (MIC) to and 1,3-β-d-glucan synthase inhibition profiles (IC₅₀) of echinocandin drugs for the strains included in the study

Strain	Genotype		Phenotype		MICc						IC50d
					ANF		CSF		MCF
	FKS1	FKS2	Fks1pa	Fks2pb	No serum	50% Serum	No serum	50% Serum	No serum	50% Serum	ANF	CSF	MCF
90030	WT	WT	WT	WT	0.06	0.50	0.06	0.12	0.03	0.25	1.91	1.92	0.71
218	WT	WT	WT	WT	0.06	0.50	0.12	0.25	0.12	1.00	1.25	3.11	0.56
3168	WT	WT	WT	WT	0.03	0.50	0.06	0.12	0.06	0.50	1.58	1.79	1.29
42997	T1874C	WT	F625S	WT	2.00	8.00	8.00	8.00	0.50	4.00	110.6	697.8	126.3
5847	T1885C	WT	S629P	WT	4.00	8.00	8.00	8.00	2.00	8.00	3855.4	4987.5	854.5
21900	A1895G	WT	D632G	WT	1.00	8.00	4.00	16.00	0.50	8.00	101.8	756.3	343.4
3169	T1896G	WT	D632E	WT	2.00	8.00	2.52	8.00	2.52	8.00	151.5	844.5	444.7
42971	G1894T	A4129T	D632Y	R1377STOP	4.00	8.00	8.00	8.00	1.00	8.00	2543.5	5248.5	413.4
31498	WT	Del1974-1976	WT	F659del	2.00	8.00	2.00	8.00	4.00	8.00	160	117.2	123.5
234	WT	T1975G	WT	F659V	1.00	8.00	4.00	8.00	1.00	8.00	86.6	115.3	113.5
41026	WT	T1976C	WT	F659S	4.00	16.00	16.00	16.00	4.00	16.00	858.1	1064.0	1067.0
3830	WT	T1987C	WT	S663P	1.00	8.00	16.00	16.00	1.00	8.00	4003.5	5115.5	2569
41400	WT	A1997G	WT	D666G	2.00	8.00	4.00	8.00	0.12	4.00	175.8	506.6	100.6
42031	WT	C1998A	WT	D666E	1.00	4.00	4.00	8.00	0.25	4.00	127.4	257.9	88.7
51916	WT	C1999A	WT	P667T	1.59	8.00	2.00	8.00	0.40	4.00	99.5	166.2	110.9
5416	WT	G4125A	WT	W1375L	0.25	4.00	4.00	8.00	0.25	2.00	85.2	608.4	50.7

^aWT, wild type at hot spots. Fks1p hot spot number one includes amino acids between 625 and 633 (625-FLILSLRDP-633).

^bWT, wild type at hot spots. Fks2p hot spot number one includes amino acids between 659 and 667 (659-FLILSLRDP-667). Fks2p hot spot number two comprises amino acids between 1374 and 1381 (WT, 1374- DWVRRYTL-1381).

^cGeometric mean values in μg ml⁻¹ (three repetitions on three separate days).

^dArithmetic mean (three repetitions on three separate days) IC₅₀s obtained using trapped 1,3-β-d-glucan synthase enzyme expressed in ng ml⁻¹.