TABLE 2.

Summary of kinetic properties of the C. glabrata 1,3-β-d-glucan synthase complexes included in the study

Strain	Genotype		Phenotype		Vmax (nmol min−1)c	K m (mM)c
	FKS1	FKS2	Fks1pa	Fks2pb
90030	WT	WT	WT	WT	7.04 ± 0.81	0.13 ± 0.02
218	WT	WT	WT	WT	6.85 ± 0.52	0.12 ± 0.04
3168	WT	WT	WT	WT	6.67 ± 0.18	0.10 ± 0.07
42997	T1874C	WT	F625S	WT	1.83 ± 0.59	0.17 ± 0.11
5847	T1885C	WT	S629P	WT	1.44 ± 0.95	0.15 ± 0.05
21900	A1895G	WT	D632G	WT	1.55 ± 0.41	0.10 ± 0.05
3169	T1896G	WT	D632E	WT	1.57 ± 0.38	0.07 ± 0.05
42971	G1894T	A4129T	D632Y	R1377STOP	1.26 ± 0.24	0.06 ± 0.01
31498	WT	Del1975-1977	WT	F659del	2.75 ± 0.22	0.10 ± 0.04
234	WT	T1975G	WT	F659V	2.87 ± 0.85	0.17 ± 0.04
41026	WT	T1976C	WT	F659S	3.05 ± 0.51	0.08 ± 0.01
3830	WT	T1987C	WT	S663P	4.45 ± 0.73	0.16 ± 0.05
41400	WT	A1997G	WT	D666G	5.05 ± 0.41	0.11 ± 0.03
42031	WT	C1998A	WT	D666E	1.85 ± 0.44	0.08 ± 0.01
51916	WT	C1999A	WT	P667T	0.33 ± 0.05	0.04 ± 0.02
5416	WT	G4125A	WT	W1375L	2.71 ± 0.09	0.04 ± 0.01

^aWT, wild type at hot spots. Fks1p hot spot number one includes amino acids between 625 and 633 (625-FLILSLRDP-633).

^bWT, wild type at hot spots. Fks2p hot spot number one includes amino acids between 659 and 667 (659-FLILSLRDP-667).

^cArithmetic mean ± standard deviation (three repetitions).