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Sia expression on type I-like pneumocytes in vitro. Primary human type I-like pneumocytes were incubated with control SAGM (left panels) or 500 U/ml of DAS181 (right panels) for 2 h at 37°C and fixed. Sia in α2-3 linkages was stained reddish brown by biotinylated MAL-I (A and B) and MAL-II (C and D) lectins, while Sia in α2-6 linkages was stained red by horseradish peroxidase-conjugated SNA (E and F). Magnification, ×400.
