TABLE 1.
Hydrogen production by JW2701-1 expressing the fhlA alleles via pCA24N after 0.5 h of anaerobic incubation in modified complex 20 mM formate medium
| Strain | fhlA allele | FhlA amino acid change(s) | na | H2 production rate (μmol mg protein−1 h−1)e | Relative H2 production rate |
|---|---|---|---|---|---|
| JW2701-1(pASKA2701) | fhlA | 24 | 0.8 ± 0.3 | 1 | |
| JW2701-1(pVSC133) | fhlA133b | Q11H, L14V, Y177F, K245R, M288K, I342F | 5 | 7 ± 2 | 9 |
| JW2701-1(pVSC14) | fhlA14c | L14G | 4 | 3.5 ± 0.5 | 4 |
| JW2701-1(pVSC1157) | fhlA1157d | M6T, S35T, L113P, S146C, E363K | 4 | 2.9 ± 0.5 | 4 |
| JW2701-1(pVSC363) | fhlA363c | E363G | 5 | 5 ± 1 | 6 |
a
n, number of independent cultures.
b
Obtained via epPCR of whole fhlA.
c
Obtained via saturation mutagenesis.
d
Obtained via epPCR of the fhlA region encoding the N domain of FhlA.
e
The values are averages ± standard deviations.