FIG. 3.

Phylogenetic tree derived from three 16S rRNA gene clone libraries constructed from bioaerosol DNA. The evolutionary distance among 16S rRNA sequences and representative members of related families is illustrated by an unrooted phylogenetic tree. The tree was inferred using the maximum-likelihood method with aligned sequences containing 900 to 950 positions. The scale bar indicates the nucleotide substitutions per position. GenBank accession numbers are given next to each species names. Experimental sequences are named as follows. In “4A_1 FJ001275 (522),” for example, the first three characters correspond to the ribotype, the numbers beside the ribotype are its GenBank accession number, and the numbers in parentheses indicate the number of clones obtained under this particular ribotype for the entire clone library.