TABLE 4.
Specificity of HumM2 and HumM3 qPCR assaysa
| Animal source | No. of animals | Avg CT (SD) for:
|
|
|---|---|---|---|
| HumM2 | HumM3 | ||
| Alpaca | 2 | ||
| Cow | 80 | ||
| Goat | 7 | ||
| Sheep | 10 | 34.1 (0.03) | |
| Horse | 12 | ||
| Pig | 22 | ||
| Antelope | 4 | ||
| Whitetail deer | 15 | ||
| Mule deer | 5 | ||
| Moose | 1 | ||
| Elk | 5 | 35.7 (0.24) | |
| Canadian goose | 12 | ||
| Duck | 12 | ||
| Pelican | 5 | ||
| Gull | 12 | ||
| Turkey | 7 | ||
| Chicken | 10 | 32.1 (0.30) | |
| Marine dolphin | 3 | ||
| California sea lion | 5 | ||
| Cat | 10 | ||
| Dog | 10 | ||
| Human | 16 | 29.7 (0.03) | 30.3 (0.10) |
| Wastewater | 20 | 31.8 (0.54) | 32.8 (1.01) |
| Total | 285 | ||
a
CT values were generated from 1 ng of total DNA.