FIG. 5.

The major pilin SpaA is colocalized with its cognate sortase SrtA and minor pilins SpaB and SpaC. Corynebacterial thin sections on nickel grids were stained with specific antiserum against SpaA (α-SpaA) and goat anti-rabbit IgG conjugated to 12-nm gold particles (filled arrows). Extensively washed samples were then reacted with α-SrtA (A and B), α-SpaB (C), or α-SpaC (D), followed by IgG-conjugated 18-nm gold particles (open arrows). Samples were viewed by transmission electron microscopy after being stained with 1% uranyl acetate. Scale bars, 0.5 μm.