FIG. 6.

Sortase and its cognate substrates colocalize with the Sec translocase. Except for the one shown in panel A, which was labeled with α-SecA and 12-nm gold particles, corynebacterial thin sections on nickel grids were stained as described in the legend to Fig. 5 with α-SecA and 18-nm gold particles (B, D, and E) or 12-nm gold particles (C), followed by α-SrtA (B and C) or α-SpaA (D and E) and 12-nm gold particles (B, D, and E) or 18-nm gold particles (C). An enlarged area of panel E (boxed) is shown in panel F. The samples were viewed by transmission electron microscopy. Scale bars, 0.2 μm.