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. 2009 Jun 19;191(18):5717–5723. doi: 10.1128/JB.00325-09

FIG. 3.

FIG. 3.

Results of left- and right-junction site-specific PCR with NRS387 and FPR3757. Lane 1 is the DNA size marker in all seven panels. The sizes of the molecular mass markers (in thousands) are indicated on the left. (A) Lane 2, lack of amplicon from primer set 1a in the NRS387 strain due to the inversion junction. Lanes 3 and 4, amplicons of 3,402 and 3,490 bp from primer sets 2b and 3c from NRS387. (B) Lanes 2 and 3, amplicons of expected sizes (3,176 and 3,330 bp) from primer sets 4d and 5e from NRS387. Lane 4, lack of amplicon from primer set 6f in NRS387. (C) Lane 2, lack of amplicon from the F/F primer combinations of 1a and 6f in the FPR3757 strain. Lane 3, An ∼4,800-bp amplicon from the F/F primer combinations of 1a and 6f in the NRS387 strain. Lane 4, lack of amplicon from R/R primer combinations of 1a and 6f in the FPR3757 strain. Lane 5, An ∼4,700-bp amplicon from the R/R primer combinations of 1a and 6f in the NRS387 strain. (D and F) PCR results from FPR3757 with primer pairs 1a5 and 6f2, respectively. (E and G) PCR results from NRS387 with the F/F and R/R combination of primer pairs 1a5 and 6f2, respectively. In panels D to G, lane 2 corresponds to FPR3757 and lane 3 corresponds to NRS387. The presence of IS1181 and 73-bp conserved sequences within the left and right junction sites is seen by the increase in size of the PCR products from NRS387 compared to those from FPR3757.