TABLE 1.
Oligonucleotide primers used to determine the left and right junctions of the inversion and sequence of the regions
| Strain or primer | Forward or reversea | Sequence (5′-3′) | PCR product size (bp) | Location of primers |
|---|---|---|---|---|
| FPR3757 | ||||
| 1a | F | CGAAGGATACGGTCCAAGAG | 3,402 | 1153285-1153304 |
| R | ACCCGGTATATGGCAATCAA | 1156667-1156686 | ||
| 2b | F | TGAAGTCCAGTGCAATTGGT | 3,875 | 1156463-1156482 |
| R | TCAAAGGAGAAATTCGAATGAA | 1160316-1160337 | ||
| 3c | F | GCCATAACGTACTGTTAGAGAAGG | 3,490 | 1160116-1160139 |
| R | TTCAGGGGGTGCACATAAAT | 1163586-1163605 | ||
| 4d | F | AGCACGTTCGAAAGGAAGAT | 3,176 | 1683350-1683369 |
| R | GTCTTGGGCCGAGAATTTTA | 1686506-1686525 | ||
| 5e | F | ATTTTCAGCCCATCAAGTGC | 3,330 | 1680210-1680229 |
| R | TGGTATGCGTTTACGTCGTG | 1683520-1683539 | ||
| 6f | F | ATCTGGCGAAGCGATTAAGA | 3,197 | 1677418-1677437 |
| R | AAGACGATGCTGAGCTTTCTG | 1680594-1680614 | ||
| NRS387 | ||||
| 1a1 | F | CGAAGGATACGGTCCAAGAG | 771 | |
| R | TTTAAGCTTCCTGCAGCTAATG | |||
| 1a2 | F | TCATCAACTGGGATAGCAACT | 1,052 | |
| R | TTGTTTGAAAAGTGTCATCATGC | |||
| 1a3 | F | TTTCCAGTTCTAGTGCTATATTGGT | 1,087 | |
| R | AGAAGTATAAGGTGGTGAACAAAAA | |||
| 1a4 | F | GGATAATCGACGTAAGAAGAATCA | 1,128 | |
| R | ACCAATTGCACTGGACTTCA | |||
| 1a5 | F | CAACTTCGTACAATATCTAGATGAACA | 379 | |
| R | TTTAGCCGATAACTTCAGAAATG | |||
| 6f1 | F | ATCTGGCGAAGCGATTAAGA | 965 | |
| R | AGTGGCGAGGTATGGGAAC | |||
| 6f2 | F | TCAATGGAACAAGTTCGTATGC | 288 | |
| R | TCGTGAATATGCAGGTATGTTTT | |||
| 6f3 | F | CAGAGCGGCATAACTGCTTA | 1,053 | |
| R | ACAAGAGATGACGCGCAAAT | |||
| 6f4 | F | GCGACTTTCATCATATAGTCACCT | 1,082 | |
| R | GATCATATGACTGAAGCGGATG | |||
| IS1181 | F | TGCAATGGCACTTTTACTGC | 593 | |
| R | TCCATCAATAGGACTTTCCAGTT |
a
F, forward; R, reverse.