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. 2009 Jul 17;191(18):5873–5876. doi: 10.1128/JB.01206-08

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FIG. 1. — SDS-PAGE monitoring of the purification process of Orf112 protein. Lane 1, broad-range protein molecular mass markers (Promega); lane 2, cell extract of non-VGJφ-infected V. cholerae 569B; lane 3, cell extract of VGJφ-infected V. cholerae 569B; lane 4, soluble fraction of the sonicate; lane 5, insoluble fraction of the sonicate; lane 6, precipitate at 30% (NH₃)₂SO₄; lane 7, supernatant at 30% (NH₃)₂SO_4; lane 8, precipitate at 50% (NH₃)₂SO₄; lane 9, supernatant at 50% (NH₃)₂SO₄; lane 10, Orf112 protein electro-eluted after preparative SDS-PAGE.