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. 2009 Jul 17;191(18):5873–5876. doi: 10.1128/JB.01206-08

FIG. 2.

FIG. 2.

Isolation and purification of RstB-His. (A) Chromatogram on a Ni-CAM HC His tag affinity column. (B) SDS-PAGE monitoring of the purification process of RstB-His. Lane 1, broad-range protein molecular mass markers (Promega); lane 2, cell extract of uninduced cultures; lane 3, cell extract of expression-induced cultures; lane 4, soluble fraction of the sonicate from expression-induced cultures; lane 5, insoluble fraction of the sonicate from expression-induced cultures; lane 6, soluble fraction of the 8 M urea extract; lane 7, RstB-His eluted from the column.