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. 2009 Jul 17;191(18):5873–5876. doi: 10.1128/JB.01206-08

FIG. 4.

FIG. 4.

Effect of salt concentration and nonrelated dsDNA competition on the binding of RstB. (A) RstB binding to phage ssDNA in the presence of 0 to 500 mM NaCl as detected by 0.5% agarose gel electrophoresis. Lane 1, 400 ng of genomic ssDNA of VGJφ (control); lane 2, same as for lane 1 plus 15.6 μg of RstB-His; lanes 3 to 7, same as for lane 2 plus 100, 200, 300, 400, and 500 mM NaCl, respectively. (B) RstB binding to replicative-form phage dsDNA in the presence of 0 to 500 mM NaCl. Lane 1, 400 ng of dsDNA of VGJφ (control); lane 2, same as for lane 1 plus 15.6 μg of RstB-His; lanes 3 to 7, same as for lane 2 plus 100, 200, 300, 400, and 500 mM NaCl. (C) DNA-binding competition by RstB. Lane 1, 400 ng of genomic ssDNA of VGJφ (control); lane 2, same as for lane 1 plus 15.6 μg of RstB-His; lanes 3 and 4, same as for lane 2 plus 500 and 1,000 ng of sheared calf thymus dsDNA, respectively; lane 5, 500 ng of calf thymus DNA (control).