TABLE 2.
Selected P. aeruginosa genes that were dysregulated in the metR mutant compared to wild-type strain PA14 under semiviscous and liquid swarming conditions
| Gene | Designation | Function | Change (fold)a | Pa |
|---|---|---|---|---|
| Regulators | ||||
| PA0248 | Probable transcriptional regulator | −2.72 | 0.03 | |
| PA0610 | prtN | Transcriptional regulator PrtN | 2.84 | 0.00 |
| PA0707 | toxR | Transcriptional regulator ToxR | −5.38 | 0.00 |
| PA0906 | Probable transcriptional regulator | 2.92 | 0.03 | |
| PA0929 | Two-component response regulator | −3.33 | 0.02 | |
| PA1637 | kdpE | Two-component response regulator KdpE | −2.37 | 0.04 |
| PA1705 | pcrG | Regulator in type III secretion | −2.19 | 0.01 |
| PA2056 | Probable transcriptional regulator | 2.16 | 0.02 | |
| PA2082 | Probable transcriptional regulator | −3.72 | 0.02 | |
| PA2337 | mtlR | Transcriptional regulator MtlR | 2.13 | 0.00 |
| PA2383 | Probable transcriptional regulator | −2.16 | 0.02 | |
| PA2577 | Probable transcriptional regulator | 2.57 | 0.00 | |
| PA2665 | Anaerobic nitric oxide reductase transcription regulator | 3.73 | 0.02 | |
| PA2846 | Probable transcriptional regulator | −2.60 | 0.03 | |
| PA2917 | Probable transcriptional regulator | −2.68 | 0.03 | |
| PA3206 | Probable two-component sensor | 2.92 | 0.00 | |
| PA3583 | glpR | Glycerol-3-phosphate regulon repressor | −2.27 | 0.00 |
| PA3830 | Probable transcriptional regulator | −3.00 | 0.00 | |
| PA5125 | ntrC | Two-component response regulator NtrC | 2.22 | 0.00 |
| PA5380 | Probable transcriptional regulator | 2.40 | 0.02 | |
| Type III secretion system and effectors | ||||
| PA0044 | exoT | Exoenzyme T | −2.08 | 0.01 |
| PA1695 | pscP | Translocation protein in type III secretion | −3.85 | 0.01 |
| PA1696 | pscO | Translocation protein in type III secretion | 21.12 | 0.00 |
| PA1699 | Conserved hypothetical protein in type III secretion | −3.57 | 0.02 | |
| PA1705 | pcrG | Regulator in type III secretion | −2.19 | 0.01 |
| PA1716 | pscC | Type III secretion outer membrane protein PscC precursor | −2.11 (2.42) | 0.00 (0.00) |
| PA1717 | pscD | Type III export protein PscD | −2.80 | 0.01 |
| PA1718 | pscE | Type III export protein PscE | −7.61 | 0.01 |
| PA1719 | pscF | Type III export protein PscF | −2.19 | 0.00 |
| PA1721 | pscH | Type III export protein PscH | −3.50 | 0.00 |
| PA1724 | pscK | Type III export protein PscK | −2.32 | 0.00 |
| Pyoveridine-related genes | ||||
| PA2385 | pvdQ | PvdQ | −18.74 | 0.00 |
| PA2386 | pvdA | l-Ornithine N5-oxygenase | −9.60 | 0.00 |
| PA2392 | pvdP | PvdP | −31.94 | 0.00 |
| PA2394 | pvdN | PvdN | −15.56 | 0.00 |
| PA2395 | pvdO | PvdO | −7.55 | 0.00 |
| PA2396 | pvdF | Pyoverdine synthetase F | −5.07 | 0.00 |
| PA2397 | pvdE | Pyoverdine biosynthesis protein PvdE | −7.66 | 0.00 |
| PA2399 | pvdD | Pyoverdine synthetase D | −5.28 | 0.02 |
| PA2400 | pvdJ | PvdJ | −10.38 | 0.00 |
| PA2402 | Probable nonribosomal peptide synthetase | −13.28 | 0.00 | |
| PA2413 | pvdH | Diaminobutyrate-2-oxoglutarate aminotransferase | −7.53 | 0.00 |
| PA2424 | pvdL | PvdL | −15.24 | 0.00 |
| PA2425 | pvdG | PvdG | −8.19 | 0.00 |
| Pyochelin-related genes | ||||
| PA4219 | Hypothetical protein | −2.79 | 0.00 | |
| PA4220 | Conserved hypothetical protein | −2.82 | 0.01 | |
| PA4221 | fptA | Fe(III)-pyochelin outer membrane receptor precursor | −6.97 | 0.01 |
| PA4222 | Probable ATP-binding component of ABC transporter | −4.06 (−3.54) | 0.00 (0.00) | |
| PA4223 | Probable ATP-binding component of ABC transporter | −3.85 (−2.77) | 0.01 (0.00) | |
| PA4224 | pchG | Pyochelin biosynthetic protein PchG | −4.67 | 0.00 |
| PA4225 | pchF | Pyochelin synthetase | −5.06 | 0.00 |
| PA4226 | pchE | Dihydroaeruginoic acid synthetase | −3.15 | 0.00 |
| PA4229 | pchC | Pyochelin biosynthetic protein PchC | −2.27 | 0.00 |
| PA4230 | pchB | Salicylate biosynthesis protein PchB | −2.83 (−2.93) | 0.00 (0.00) |
| PA4231 | pchA | Salicylate biosynthesis isochorismate synthase | −3.45 | 0.00 |
| Bacteriophage Pf1 genes | ||||
| PA0717 | Hypothetical protein of bacteriophage Pf1 | 342.12 (10.08) | 0.00 (0.00) | |
| PA0718 | Hypothetical protein of bacteriophage Pf1 | 271.30 (5.74) | 0.00 (0.00) | |
| PA0719 | Hypothetical protein of bacteriophage Pf1 | 109.98 | 0.00 | |
| PA0720 | Helix-destabilizing protein of bacteriophage Pf1 | 325.95 (24.11) | 0.00 (0.00) | |
| PA0721 | Hypothetical protein of bacteriophage Pf1 | 375.78 | 0.00 | |
| PA0725 | Hypothetical protein of bacteriophage Pf1 | 7.01 (3.69) | 0.00 (0.00) | |
| PA0726 | Hypothetical protein of bacteriophage Pf1 | 47.72 | 0.00 | |
| PA0727 | Hypothetical protein from bacteriophage Pf1 | 11.96 | 0.00 |
a
The values not in parentheses are data for semiviscous swarming conditions, and the values in parentheses are data for liquid swarming conditions.