FIG. 2.

Effect of recycling and nagE mutations on levels of GlcNAc plus GlcNAc6P and nagB-lacZ expression. (A) Soluble extracts of strains belonging to the different mutant groups (see panel B) were tested to determine the levels of GlcNAc plus GlcNAc6P by the modified Morgan-Elson method. Values for different strains belonging to the same mutant group are combined, and the results are the means and standard deviations for between 2 to 10 independent cultures. (B) Genotypes of strains in the different mutant groups. (C) β-Galactosidase activities of the nagB-lacZ fusion in strains belonging to mutant groups 1 to 8 and the isogenic manXYZ groups 1m to 9m. The data are the means and standard deviations for 2 to 10 independent cultures. (D) Total GlcNAc reacting material was measured by the Morgan-Elson method in soluble extracts of mutant group 1 to 8 strains and the isogenic manXYZ strains (mutant groups 1m to 8m) before and after treatment of the extracts with NagA (GlcNAc6P deacetylase). The first bar in each set of four bars indicates the total amount of GlcNAc plus GlcNAc6P in the manXYZ⁺ strain; the second bar indicates the amount of GlcNAc6P in the manXYZ⁺ strain; the third bar indicates the total amount of GlcNAc plus GlcNAc6P in the manXYZ strain; and the fourth bar indicates the amount of GlcNAc6P in the manXYZ strain. A subset of the extracts tested to obtain the data in panel A were reanalyzed in this test. The data are the means and standard deviations for two to six independent cultures. 5OD, 5 OD₆₅₀ units.