TABLE 1.
Bacterial strainsa
| Strain | Relevant genotype | Reference(s) | Mutant group |
|---|---|---|---|
| Strains with mutations in PTS and PG-recycling genes | |||
| DY nagA::FRTcm | nagA::FRTcm | ||
| DY nagA::FRTkan | nagA::FRTkan | ||
| IBPC534 | nagA::tc534 | 25 | |
| HfrH01 | ampG::kan | 10 | |
| TP77 | nagZ::cm | 2 | |
| TB71BK | nagK::FRTcm | 29 | |
| TP71B anmK | anmK::FRTcm | 31 | |
| TJ2 | murQ::FRTkan | 12 | |
| MG1655 murP | murP::FRTkan | 4 | |
| JW0662 | nagE::FRTkan | 1 | |
| DY manXYZ::FRTcm | manXYZ::FRTcm | ||
| IBPC707 | manXYZ::Tn9 | 26 | |
| IBPC590 | ΔnagEBACD::tc | 23, 24 | |
| Strains analyzed | |||
| MC-B1 | MC4100 λRS/nagE-B | B | |
| MC-B11 | nagA::tc534 | 1 | |
| MC-B31 | nagA::FRTkan | 1 | |
| MC-B48 | nagKmurQ anmK ΔnagEBACD::tc | 11 | |
| MC-B51 | nagKmurQ anmKnagA::FRTkan | 3 | |
| MC-B52 | ΔnagEBACD::tc | 10 | |
| MC-B61 | nagA::FRTcm | 1 | |
| MC-B62 | nagKmurQ anmK ampG::kan nagZ::cm | B | |
| MC-B71 | manXYZ::FRTcmnagE::FRTkan | B | |
| MC-B90 | nagE::FRTkannagA::FRTcm | 5 | |
| MC-B120 | nagKmurQ anmK ampG::kan nagZ::cm ΔnagEBACD::tc | 12 | |
| MC-B123 | nagKmurQ anmK nagZ::cm nagA ampG::kan | 4 | |
| MC-B124 | nagKmurQ anmK ampG::kan nagA nagZ::cm | 4 | |
| MC-B125 | manXYZnagE::FRTkan nagA::FRTcm | 5m | |
| MC-B128 | nagE::FRT kannagA::FRTcm | 5 | |
| MC-B134-1P | nagKmurQ anmK manXYZ nagE nagB nagA ampG::kannagZ::cm | 8m | |
| MC-B134-2R | murQanmK manXYZnagEnagB nagA ampG::kan nagZ::cm | 9m | |
| MC-B137 | nagKmurQ anmK manXYZ nagE ampG::kan nagZ::cm | B | |
| MC-B139 | nagKmurQ anmK manXYZ::FRTcmnagA::FRTkan | 3m | |
| MC-B141 | manXYZnagE nagB nagA ampG::kan nagZ::cm | 6m | |
| MC-B144 | nagKmurQ anmK nagE::FRTkannagA::FRTcm | 7 | |
| MC-B157 | ampG::kannagZ::cm nagA::tc534 | 2 | |
| MC-B158 | ampG::kannagA nagZ::cm | 2 | |
| MC-B159 | nagKmurQ anmK nagA::FRTcm | 3 | |
| MC-B160 | nagKmurQ anmK nagE::FRTkannagA::FRTcm | 7 | |
| MC-B162 | nagKmurQ anmK manXYZnagE::FRTkannagA::FRTcm | 7m | |
| MC-B163 | nagKmurQ anmK nagA::tc534 | 3 | |
| MC-B166 | nagKmurQ anmK manXYZ nagE::FRTkannagA::tc534 | 7m | |
| MC-B170 | nagKmurQ anmK nagE nagB nagA manXYZ::FRTcm | 7m | |
| MC-B171 | nagE::FRTkannagA::FRTcm | 5 | |
| MC-B174 | nagA::FRTcm | 1 | |
| MC-B174C | nagA | 1 | |
| MC-B175 | nagE::FRTkannagA::FRTcm | 5 | |
| MC-B175C | nagEnagB nagA | 5 | |
| MC-B177 | nagA::FRTcm | 1m | |
| MC-B178 | nagE::FRTkannagA::FRTcm | 5m | |
| MC-B179 | nagE::FRTkannagA::FRTcm | 5m | |
| MC-B180 | nagAmanXZ::FRTcm | 1m | |
| MC-B181 | nagEnagB nagA manXZ::FRTcm | 5m | |
| MC-B182 | nagAmanXYZ::Tn9 | 1m | |
| MC-B204 | nagEnagB nagA ampG::kan nagZ::cm | 6 | |
| MC-B205 | nagKmurQ anmK nagE nagB nagA nagZ::cm ampG::kan | 8 | |
| MC-B206 | nagKmurQ anmK manXYZ nagE nagB nagA nagZ::cm ampG::kan | 8m | |
| MC-B210 | manXYZnagA ampG::kan nagZ::cm | 2m | |
| MC-B212 | nagAampG::kan nagZ::cm | 2 | |
| MC-B221 | nagKmurQ anmK nagA ampG::kan nagZ::cm | 4 | |
| MC-B222 | nagKmurQ anmK manXYZ nagA ampG::kan nagZ::cm | 4m | |
| MC-B223 | nagEnagB nagA ampG::kan nagZ::cm | 6 | |
| MC-B224 | manXZnagE nagB nagA ampG::kan nagZ::cm | 6m | |
| MC-B225 | nagKmurQ anmKnagA::FRTkanmanXYZ::FRTcm | 3m | |
| MC-B265 | murPnagA::FRTcm | ||
| MC-B270 | murPmanXYZ nagA::FRTcm | ||
| MC-B275 | murPnagE::FRTkannagA::FRTcm | ||
| MC-B280 | murPmanXYZ nagE::FRTkannagA::FRTcm |
All strains whose designation begins with MC-B are derivatives of MC-B1 (MC4100 carrying a nagB-lacZ fusion on a λ lysogen). Strains are divided into mutant groups based on the set of mutations carried (Fig. 2B). The manXYZ derivatives of the mutant groups are indicated by the suffix m. Mutant group B contains the nagA+ strains tested in the experiments whose results are shown in Table 2. The strains in a group can carry the same or different alleles introduced in a different order and/or cured for the antibiotic cassette. Mutations are listed in the order in which they were introduced. FRT indicates that the antibiotic resistance cassette is surrounded by FRT sites. The suffix C in a strain designation indicates that the antibiotic cassette was cured by transformation with pCP20 (5).