FIG. 4.
Processivity of Cel5H activity. Purified Cel5H was incubated with filter paper for the indicated times, and products formed as reducing sugar were determined as described in Materials and Methods. Diamonds (top line) indicate soluble reducing sugar detected (μmol cellobiose). Squares (bottom line) indicate insoluble reducing sugar (μmol glucose). Linear trend lines were calculated using y = mx. For release of soluble reducing sugar, m is 0.0108, with a goodness of fit R2 value of 0.97. For formation of insoluble reducing sugar, m is 0.0020 and R2 is 0.94.