FIG. 5.

COMSTAT and statistical analyses of biomass (A) and average thickness (B) for wild-type (checkered bars) and aniA::kan (black bars), ccp::kan (gray bars), and norB::kan (white bars) insertion mutant biofilms grown over THCEC for 2 days. Representative images of these biofilms are depicted in Fig. 4. All strains were run in duplicate in a minimum of three experiments, and at least four images of each biofilm chamber were used for COMSTAT analysis. The error bars represent 1 standard deviation from the mean. Statistical differences between mutants and the wild type, determined by Student's t test, are denoted by asterisks above the error bars. *, P value of 0.05 or less; **, P value of 0.01 or less.