TABLE 1.
Primers used in this study
| Primera | Target | Sequence (5′-3′) |
|---|---|---|
| qRT-PCR primers | ||
| MFRTF8 | omp3 | CGTCGGCATCGCTTTTG |
| MFRTR8 | omp3 | CAGGCTGTTCATGCGGTAGTC |
| MFRTF27 | secD | ACCAACCGACAAGCCATCAT |
| MFRTR27 | secD | CAATCGACCGCGACTATCCT |
| MFRTF28 | aniA | CAATCGACCGCGACTATCCT |
| MFRTR28 | aniA | GGTTTTTTCGACGGTTTCCA |
| MFRTF29 | ccp | GCGCAAGGTGTATTCCAACCT |
| MFRTR29 | ccp | AACGGACGGATTTTCTGCAT |
| MFRTF30 | fimT | GACAAAAACGGCAATAAGGAATATG |
| MFRTR30 | fimT | CGCTGCGGAGGAAAACAT |
| MFRTF31 | nifU | TTCCGCCATCGCTTCGT |
| MFRTR31 | nifU | TCATCCAGACTTTTGCCTTTG |
| MFRTF32 | nuoF | GCATTGCTTGAATCGTTGGAA |
| MFRTR32 | nuoF | GGGAACGGCGGTTTGAA |
| MFRTF33 | opaB | CAACCGCTCCAGGCAAAA |
| MFRTR33 | opaB | TGCGTACGGATGTTTCTGAAAT |
| MFRTF35 | tatC | AGACCCTGCTTGCCATTCC |
| MFRTR35 | tatC | AGCGTCCGAACCAAATGC |
| MFRTF36 | ngo0023 | TTGAGCCCGATTGCGAAT |
| MFRTR36 | ngo0023 | CCGCCGGTAATGACAAACTG |
| MFRTF37 | modA13 | CGAGCAAGGCGGTATCTCAA |
| MFRTR37 | modA13 | GGAAAGTTCGGCATAAACAAACTC |
| MFRTF38 | norB | CGAAAGCATCCTGCCTTACTATC |
| MFRTR38 | norB | GCGGGTGGTTTGCAACTT |
| MFRTF39 | secF | GCAGGGTGCGGATGTCA |
| MFRTR39 | secF | CACCCATTTTCAGCGTATCGA |
| MFRTF40 | tatB | TTTGGGCGAGCTGATTTTTG |
| MFRTR40 | tatB | GGCGTTCTGGACCAAGGA |
| MFRTF41 | narP | CCGCAGCAGGCAGTCATT |
| MFRTR41 | narP | CGGTAAGGTCGTCGCTGTCT |
| MFRTF42 | narQ | TGCAGGAGCGTGCCAAA |
| MFRTR42 | narQ | TTGTGCTTGGGAACGGATTT |
| MFRTF43 | mucD | GCCGGTATGGGCAGTATCAA |
| MFRTR43 | mucD | CCGATGAGTTTGGCGGTATATT |
| Cloning primers | ||
| aniA_F | ATGAAACGCCAAGCCTTAG | |
| aniA_R | TAAACGCTTTTTTCGGATGCAG | |
| aniA_5′Xho1F | ACCGCTCGAGGCCGTACTTCCACATTCA | |
| aniA_5′Xho1R | TGAATGTGGAAGTACGGCCTCGAGCGGT | |
| aniA_upstm | AACTACCCTGCCTTTGCCTGATT | |
| aniA_downstm | CCGAGGAAAAATAACCGGACATAC | |
| NorBF | CAGAGCAGGCAAAGGCAG | |
| NorBRev | GAACAGCCCTACCGCATC |
a
An F as the last letter of the name denotes a forward primer, while an R denotes a reverse primer. For the cloning primers, the primer name denotes the target.