TABLE 3.

qRT-PCR validation of microarray results^a

Gene (KEGG accession no.)	Gene name	Fold change in array assay (biofilm/planktonic growth)	Fold change by qRT-PCR (biofilm/ planktonic growth) (mean ± SD)
Upregulated genes meeting a 2.0-fold cutoff
NGO0641	modA13	2.3	1.4 ± 0.2
NGO1769	ccp	2.6	6.6 ± 1.6
NGO0070	opaB	2.8	1.7 ± 1.3
NGO1275	norB	2.8	6.7 ± 2.0
NGO1276	aniA	5.4	17.9 ± 4.2
Upregulated gene not meeting a 2.0-fold cutoff
NGO0452	fimT	1.8	1.3 ± 0.3
Downregulated genes meeting a 2.0-fold cutoff
NGO1746	nuoF	2.1	3.5 ± 0.8
NGO0182	tatB	2.2	2.6 ± 0.6
NGO0752	narL	2.4	3.1 ± 0.6
NGO0023		5.1	1.3 ± 0.3
Downregulated genes not meeting a 2.0-fold cutoff
NGO0189	secD	1.5	2.6 ± 1.3
NGO0190	secF	1.5	3.0 ± 1.4
NGO0138	mucD	1.6	3.5 ± 1.2
NGO0181	tatC	1.7	1.9 ± 0.3
NGO0633	nifU	1.7	4.7 ± 0.8
NGO0753	narX	1.9	2.5 ± 0.3

^aGenes shown in bold did not meet our criteria for validation.