FIG. 4.

Effects of S. gordonii on C. albicans signaling pathways. (A and B) C. albicans suspensions in YPT-Glc incubated for 4 h at 37°C, showing inhibition of hyphal formation by 30 μM farnesol (A) and suppression of 30 μM farnesol inhibition by S. gordonii DL1 (B). (C) Western immunoblot analysis of effects of 10 mM H₂O₂ or 0.1 mM farnesol (Fnl) on phosphorylation of C. albicans MAP kinases after 20 min in the absence (left three lanes) or presence (right three lanes) of S. gordonii DL1. MAP kinases were detected with anti-phospho-p44/42 MAP kinase antibody (Mkc1p, Cek1p, Cek2p), anti-phospho-p38 MAP kinase antibody (Hog1p), and an anti-ScHog1 polyclonal antibody (Hog1) control. Experiments were repeated three times, and representative data from one experiment are shown.