FIG. 4.
Effect of l-NMMA on T. gondii growth in response to TgCyp18. Peritoneal macrophages (1 × 106 macrophages) from B6 mice were pretreated with 0 μM, 500 μM, or 1,000 μM l-NMMA for 15 min. The cells were incubated with TgCyp18, MTgCyp18, 50 μg/ml GST, or 10 ng/ml LPS for 12 h before infection with 2 × 105 parasites. After incubation for 24 h, the [3H]uracil uptake in tachyzoites was measured. The percentage of growth was calculated by dividing each value (control or tested) by the average means of the control samples (parasite growth in nontreated macrophages) multiplied by 100. Each value represents the mean ± standard deviation of data from triplicate samples. Statistical significance was calculated against 0 μM l-NMMA treatment using ANOVA and a follow-up test (LSD). *, P < 0.001.