FIG. 6.

IFAT of T. gondii-infected macrophages pretreated with TgCyp18. Peritoneal macrophages (1 × 10⁶ macrophages) were incubated with TgCyp18 or MTgCyp18 (50 μg/ml) or pretreated with 1,000 μM l-NMMA for 15 min, followed by 50 μg/ml of TgCyp18 for 12 h before the culture was infected with T. gondii (2 × 10⁴ parasites). After 72 h, the cells containing the parasite were subjected to IFAT. Cells treated with TgCyp18 (A, E, I, and M), TgCyp18 plus l-NMMA (B, F, J, and N), MTgCyp18 (C, G, K, and O), or complete medium (D, H, L, and P) were stained with a BAG1 antibody as a bradyzoite-specific marker (A to D) or a TgCyp18 antibody as a tachyzoite and bradyzoite marker (E to H). Light images (I to L) and merged images (M to P) are also shown. For the conversion ratio of T. gondii from the tachyzoite stage to the bradyzoite stage (Q), the data are presented as mean percentages of duplicated samples of BAG1-positive and TgCyp18-positive cells of 300 cells infected with T. gondii. Scale bars, 10 μm.