FIG. 1.
Sequence homology and expression analysis of the A. fumigatus prtT (AFUA_4G10120) gene. (A and B) A. fumigatus PrtT contains an N-terminal 29-amino-acid (aa) fungal C6 Zn finger domain (underlined) (A) and is conserved among Aspergillus and Penicillium species as show in the dendrogram (B). (C) Expression of A. fumigatus prtT. Expression was determined by RT-PCR using primers AfPrtT inner 5′ and 3′ (Table 1). Dormant conidia were incubated for 0.5, 1, 2, and 3 days in liquid SM medium at 37°C, after which total RNA was isolated and used in RT-PCR. RT-PCR with primers for gpdA, a housekeeping gene, was used as a control for the loading (lower bands). A control PCR, performed on RNA following DNase treatment, gave no visible band (data not shown). In lane 1+ NH4, the conidia were incubated in SM in the presence of 5 mM ammonia. This experiment was independently performed twice with similar results.