TABLE 1.

Primers used in this study

Primer name	Sequence (5′ → 3′)
PrtT outer 5′	ATGGCGCGCCGCACTGATCTCGTCCTATCGGTT
PrtT outer 3′	ATGGCGCGCCCTAAAGTCTCCTACCTACCTCAG
Hyg 5′	ATAAGCTTGTCGACAGAAGATGATAT
Hyg 3′	ATAAGCTTGCTCTCCCTTATGCGACTCCTGCA
PrtT up 3′	AGTGACAGAACAGGCGACAG
pAN-8.1 NdeI 5′	ATCATATGGAATTCCCTTGTATCTCTACACAC
pAN-8.1 NdeI 3′	TGCACCATATGCGGTGTGAAATA
gpdA 5′	TCTCCAACGTTCTTGCACC
gpdA 3′	CCACTCGTTGTCGTACCAGG
prtT inner 5′	GGTGATAGCGAAACAGAAGAGACAAG
prtT inner 3′	ATAGACTCCACTGGTAGTTAGGTGTTCTG
AFUA_8G07080 (Mep) 5′	AACTTTGCCACTCCTCCTGA
AFUA_8G07080 (Mep) 3′	GCCGTGCTTATCGATCAAAT
AFUA_4G11800 (Alp1) 5′	GCATCAATGTCAACCACGTC
AFUA_4G11800 (Alp1) 3′	CATCGAAAGCGTTCTCAACA
AFUA_6G00310 (CpdS) 5′	AGCTGGTTCAAGCACTTCGT
AFUA_6G00310 (CpdS) 3′	TGAAGGGCTTTTTGGACATC
AFUA_4G09320 (DppIV) 5′	AAGGCCTACATTGCCTCAGA
AFUA_4G09320 (DppIV) 3′	CTCCTCGTACAACCTCTTGG
AFUA_2G17330 5′	CACCGAGGCCATTGTAGATT
AFUA_2G17330 3′	TAACCCCAGACGATTTTTGC
AFUA_7G06220 5′	TACTATCGGGGCCAGTTACG
AFUA_7G06220 3′	AGAGAGCTCCTGGGTTGGAT
AFUA_4G03790 5′	ATCCTGGCTCAGTCTGCTGT
AFUA_4G03790 3′	CGAACGTCCTTTTGTTGGAT