TABLE 2.

Protease activities of the WT, mutant (prtTΔ), and reconstituted (KI) strains^a

Substrateb	Total absorbance units/g (dry wt) myceliumc
	WT	prtTstrain	KI strain
SM	9.6 ± 1.4	0.6 ± 0.1	10.7 ± 0.9
SM without glucose	8.7 ± 0.1	1.7 ± 0.02	10.0 ± 0.5
SM without nitrogend	10.8 ± 0.7	1.3 ± 0.3	12.9 ± 0.7
SM without phosphatee	22.4 ± 1.2	1.1 ± 1	19.9 ± 1.6
SM without sulfurf	4.5 ± 2.5	0.3 ± 0.2	7.4 ± 1.1
SM without nitrogen and phosphate	22 ± 1	0.6 ± 0.4	21.6 ± 0.4
SM without phosphate and sulfur	18 ± 1.2	0.7 ± 0.4	21.8 ± 1.1
SM without nitrogen, phosphate, and sulfur	20.7 ± 0.5	0.5 ± 0.1	21 ± 0.5
SM + NH4	0.6 ± 0.03	0.6 ± 0.05	0.3 ± 0.02
SM, pH 5.3	11.8 ± 0.1	0.8 ± 0.8	13.3 ± 0.9
SM, pH 7	7.5 ± 0.1	0.3 ± 0.3	9.1 ± 1.1
SM, pH 8	4.6 ± 0.4	0.2 ± 0.2	8.3 ± 0.8

^aThe protease activity of CFs collected after 48 h of growth on various liquid media was measured by azocasein release (see Materials and Methods).

^bSM was at pH 6.2 unless otherwise indicated.

^cResults are expressed in arbitrary units and results are the averages and standard deviations for three to six cultures.

^dWithout Casamino Acids as a nitrogen source.

^eWithout Na₂HPO₄/NaH₂PO₄ as phosphate sources.

^fWithout MgSO₄ as a source of sulfur.