Refolded and purified rDBLα minimal domain. (A) Mobility of refolded and purified DBLα by SDS-PAGE before and after reduction with β-mercaptoethanol (β-ME). (B) Silver-stained SDS-PAGE gel of refolded and purified rDBLα. Different quantities (0.5, 1.0, 1.5, and 2.0 μg) of refolded and purified rDBLα were reduced, denatured, separated by SDS-PAGE, and detected by silver staining. (C) Reverse-phase chromatography profile of refolded and purified rDBLα.