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TTSS-mediated effect on virulence of B. bronchiseptica strain S308, a ST32 isolate. Groups of three or four C57BL/6 mice were inoculated intranasally with the indicated doses of strain S308 or S308ΔbscN. Survival curves were generated by inoculating mice with the indicated dose and determining the percent survival over a 28-day period.
