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Activation of GSK-3β in heat-inactivated S. aureus-stimulated microglia. BV-2 mouse microglial cells (2.5 × 105) were treated with heat-inactivated S. aureus (2.5 × 106) for the different time periods, as indicated. Western blotting was used to determine phosphorylation of GSK-3β (Ser9 and Tyr216) and GS (Ser641). β-Actin was the internal control. Data shown are representative of the results for three individual experiments.
