FIG. 2.

Activation of the AP of complement on Loa loa MF in vitro. Patient blood-isolated Loa loa MF, complement-activating zymosan A yeast particles (ZymA), and complement-nonactivating human red blood cells (RBC) were exposed to increasing concentrations of NHS. The amount of surface-bound C3b and iC3b molecules was measured by staining the cells or MF with rabbit anti-C3c antibody and Alexa 488-conjugated secondary antibody followed by detection of the bound fluorescence using a fluorometer. The fluorescence intensity obtained without any NHS incubation was subtracted as background. The results are shown as means for parallel samples assayed in duplicate.