FIG. 4.

Analysis of cofactor activity of Loa loa MF-bound CFH. The MF were isolated from EDTA-anticoagulated blood by the Percoll gradient method and washed. The cofactor activity of the MF-bound CFH was analyzed by incubating the MF with ¹²⁵I-labeled C3b and FI (C3b+FI+MF). The positive control sample contained ¹²⁵I-labeled C3b, FI, and CFH and the negative control only ¹²⁵I-labeled C3b and FI. The mobility of the molecular weight markers is shown on the left, and the identities of the fragments of C3b are indicated on the right.