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Loa loa MF bind purified CFH and C4BP. The MF were isolated from EDTA-anticoagulated blood (40 MF/tube) or heparin-anticoagulated blood (100 MF/tube) and incubated with 125I-labeled CFH, C4BP, or BSA for 30 min. The unbound proteins were separated from the bound ones by centrifugation through 20% sucrose. Radioactivity in the pellets and supernatants was measured using a gamma counter. The experiment was performed twice in duplicate, and the mean values are indicated.
