FIG. 4.

Actin polarization defects of rvs161Δ and rvs167Δ strains. Actin localization in budding cells (A) and cells induced to form hyphae (B) by growth in medium containing 10% bovine calf serum at 37°C. Wild-type strain DIC185 and the indicated rvsΔ mutant strains were analyzed by staining fixed cells with rhodamine-conjugated phalloidin (top panels) and by DIC light microscopy (bottom panels). The deletion of RVS161 and RVS167 resulted in the increased appearance of actin patches on the mother cell surface with few cables. The rvs162Δ strain appeared similar to the wild-type and complemented strains in which the corresponding wild-type RVS gene was reintroduced. Strains used were YLD14 (rvs161Δ), YLD22 (rvs162Δ), YLD16 (rvs167Δ), YLD11 (rvs161Δ/rvs161Δ/RVS161), and YLD12 (rvs167Δ/rvs167Δ/RVS167).