TABLE 2.
Condition | Wild type | rvs161Δ | rvs162Δ | rvs167Δ |
---|---|---|---|---|
NaCl (1.5 M) | +++ | +++ | +++ | +++ |
CaCl2 (0.5 M) | +++ | +++ | +++ | +++ |
LiCl (0.15 M) | +++ | − | +++ | − |
Calcofluor white (40 μg/ml) | +++ | + | +++ | ++ |
Congo red (100 μg/ml) | +++ | + | +++ | + |
Sodium dodecyl sulfate (0.06%) | +++ | ++ | +++ | +++ |
HEPES (pH 8.5; 150 mM) | +++ | ++ | +++ | ++/+++ |
H2O2 (5 mM) | +++ | ++ | +++ | + |
8-wk starvationb | +++ | +++ | +++ | +++ |
Dilutions of cells were spotted on the indicated plates, and then relative growth was recorded. Growth relative to the wild-type strain under each condition is indicated as follows: +++, >30% of that of the wild type; ++, 3% to 30% of that of the wild type; +, 0.3% to 3% of that of the wild type; and −, no growth.
wk, week. rvs161Δ grew more slowly coming out of the stationary phase but did not display reduced viability.