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Dose-dependent induction of C5a peptidase transcription in S. agalactiae strain AC475 harboring plasmid pBSU409scpBProm. Bacteria were cultivated overnight at 37°C in THY broth supplemented with human serum in concentrations as indicated. Fetal calf serum served as a control. Shown are mean fluorescence values and standard deviations from five independent experiments as measured by FACS analysis. HS, human serum; FCS, fetal calf serum.
