TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| E. coli DH5α | endA1 hsdR17 supE44 ΔlacU169 (φ80lacZΔM15) recA1 gyrA96 thi-1 relA1 | Boehringer Ingelheim |
| S. agalactiae strains | ||
| O90R (ATCC 12386) | R. Lancefield grouping strain, human isolate | American Type Culture Collection |
| AC475 | Clinical isolate | 35 |
| BSU147 | Clinical isolate | 7 |
| BSU191 | Clinical isolate | 7 |
| BSU77 | Bovine isolate (Gi11d) | Gießen collection (C. Lämmler) |
| O90RΔscpBΔlmb | O90R derivative, scpB-lmb deletion mutant | 17 |
| O90RΔrgfC | O90R derivative, rgfC deletion mutant | This study |
| O90RpBSU409 | O90R derivative harboring pBSU409 without insert | This study |
| O90RscpBProm | O90R derivative harboring pBSU409scpBProm | This study |
| O90RlmbProm | O90R derivative harboring pBSU409lmbProm | This study |
| S. pyogenes BSU316 | Clinical isolate | Ulm collection |
| Plasmids | ||
| pAT28 | Sprori pUC ori pAmβ1 | 43 |
| pEGFP | Amr, source of egfp | Clontech Laboratories |
| pBSU409 | pAT28 derivative, novel MCS, carrying a promotorless egfp gene | This study |
| pBSU409scpBProm | pBSU409 derivative carrying the promoter region of scpB | This study |
| pBSU409lmbProm | pBSU409 derivative carrying the promoter region of lmb | This study |
a
Sp, spectinomycin; Am, ampicillin.