TABLE 2.

Oligonucleotide primers and LightCycler hybridization probes

Primer	Descriptiona	Sequenceb
pAT28-3	Control of recombination	5′ GTTGTGTGGAATTGTGAGCGG 3′
pAT28-EGFP4	Control of recombination	5′ CCTTGAAGAAGATGGTGCGC 3′
pAT28-EGFP5	Construction of the novel MCS	5′ CAGGCCTACTAGTCGTACG 3′
pAT28-EGFP6	Construction of the novel MCS	5′ GATCCGTACGACTAGTAGGCCTGAGCT 3′
scpBProm1	Construction of O90RscpBProm	5′ GCGCCGGAATTCGGAGGTCACAAAATAAGCAACTC 3′
scpBProm2	Construction of O90RscpBProm	5′ GCGCGGATCCGTGTCGTCCTTTCATGATG 3′
lmbProm1	Construction of O90RlmbProm	5′ GCGCCGGAATTCGTATTACTTAAACTGATAAGTAGC 3′
lmbProm2	Construction of O90RlmbProm	5′ GCGCGGATCCGTACCTCCTCAATTATAATTTAACC 3′
rgfCdel1	Construction of rgfC deletion	5′ TGGCACAAGCTTGAAGCATTTAGCAGAAGG 3′
rgfCdel2	Construction of rgfC deletion	5′ CCCATCCACTAAACTTAAACAAAGCTATCTCTAAGATGG 3′
rgfCdel3	Construction of rgfC deletion	5′ TGTTTAAGTTTAGTGGATGGGCTATTGCAACAAGTAGCC3′
rgfCdel4	Construction of rgfC deletion	5′ CCGCGGATCCTAGAAACCATTGGACGCG 3′
scpBLC1for	RT-PCR experiments, scpB	5′ ACCAGCCATATCTTCAACAACA 3′
scpBLC1rev	RT-PCR experiments, scpB	5′ AACATCGGCAACATTCTCAAC 3′
lmbLC1for	RT-PCR experiments, lmb	5′ TTCGCAATAGCATGAGCAA 3′
lmbLC1rev	RT-PCR experiments, lmb	5′ AGAAGCAGAGCAACTAACTGAAGA 3′
gyrA-F	LightCycler primer, reference gene, gyrA in GBS	5′ CCTGGTCCAGATTTTCCTAC 3′
gyrA-R	LightCycler primer, reference gene, gyrA in GBS	5′ TGCGCTTTTCTTGTGCTA 3′
gyrA-FL	LightCycler hybridization probe, reference gene, gyrA in GBS	CCAGTTTCATAGGCACGGTGAA-FL
gyrA-LC	LightCycler hybridization probe, reference gene, gyrA in GBS	R640-ACCTGAACGTCCCATCACCAAG-PH
gyrA-R2	LightCycler primer, reference gene, gyrA in GAS	5′ CACTCCTTCACGGCTAGATT 3′
gyrA S	LightCycler primer, reference gene, gyrA in GAS	5′ CTGGACCTGACTTTCCG 3′
gyrA-FL2	LightCycler hybridization probe, reference gene, gyrA in GAS	ATTGACGCCATAGGGAAATTCAGTAACC-FL
gyrA-LC2	LightCycler hybridization probe, reference gene, gyrA in GAS	R640-CAATACGTTCCCGACCTGTTTGAGTTG-PH
scpB-se	LightCycler primer, scpB	5′ CAGCTGAGGTCACAATGCTAAC 3′
scpB-as	LightCycler primer, scpB	5′ GCTCAAGCTATCAGAGATGCTGTC 3′
scpB-FL	LightCycler hybridization probe, scpB	GGTTGGCGTAAGCTAGTGCAGCA-FL
scpB-LC	LightCycler hybridization probe, scpB	R640-TACCAAAGCTCATATTAATCACCTTAGCTCCC-PH
lmb-fw	LightCycler primer, lmb	5′ GCCTTGTGTGACTTCCATATCTT 3′
lmb-as	LightCycler primer, lmb	5′ TGATGTGAGGATGATCCAATCAG 3′
lmb-FL	LightCycler hybridization probe, lmb	CGCGTCATAAATAGCTGCCACATCATTTACA-FL
lmb-LC	LightCycler hybridization probe, lmb	R640-ACGGTTCAAAGGAATGAATGCCTGCA-PH

^aGAS, group A streptococci.

^bFL, fluorescein; R640, LightCycler Red640 N-succinimide ester; PH, 3′ phosphate.