TABLE 2.
Primer | Descriptiona | Sequenceb |
---|---|---|
pAT28-3 | Control of recombination | 5′ GTTGTGTGGAATTGTGAGCGG 3′ |
pAT28-EGFP4 | Control of recombination | 5′ CCTTGAAGAAGATGGTGCGC 3′ |
pAT28-EGFP5 | Construction of the novel MCS | 5′ CAGGCCTACTAGTCGTACG 3′ |
pAT28-EGFP6 | Construction of the novel MCS | 5′ GATCCGTACGACTAGTAGGCCTGAGCT 3′ |
scpBProm1 | Construction of O90RscpBProm | 5′ GCGCCGGAATTCGGAGGTCACAAAATAAGCAACTC 3′ |
scpBProm2 | Construction of O90RscpBProm | 5′ GCGCGGATCCGTGTCGTCCTTTCATGATG 3′ |
lmbProm1 | Construction of O90RlmbProm | 5′ GCGCCGGAATTCGTATTACTTAAACTGATAAGTAGC 3′ |
lmbProm2 | Construction of O90RlmbProm | 5′ GCGCGGATCCGTACCTCCTCAATTATAATTTAACC 3′ |
rgfCdel1 | Construction of rgfC deletion | 5′ TGGCACAAGCTTGAAGCATTTAGCAGAAGG 3′ |
rgfCdel2 | Construction of rgfC deletion | 5′ CCCATCCACTAAACTTAAACAAAGCTATCTCTAAGATGG 3′ |
rgfCdel3 | Construction of rgfC deletion | 5′ TGTTTAAGTTTAGTGGATGGGCTATTGCAACAAGTAGCC3′ |
rgfCdel4 | Construction of rgfC deletion | 5′ CCGCGGATCCTAGAAACCATTGGACGCG 3′ |
scpBLC1for | RT-PCR experiments, scpB | 5′ ACCAGCCATATCTTCAACAACA 3′ |
scpBLC1rev | RT-PCR experiments, scpB | 5′ AACATCGGCAACATTCTCAAC 3′ |
lmbLC1for | RT-PCR experiments, lmb | 5′ TTCGCAATAGCATGAGCAA 3′ |
lmbLC1rev | RT-PCR experiments, lmb | 5′ AGAAGCAGAGCAACTAACTGAAGA 3′ |
gyrA-F | LightCycler primer, reference gene, gyrA in GBS | 5′ CCTGGTCCAGATTTTCCTAC 3′ |
gyrA-R | LightCycler primer, reference gene, gyrA in GBS | 5′ TGCGCTTTTCTTGTGCTA 3′ |
gyrA-FL | LightCycler hybridization probe, reference gene, gyrA in GBS | CCAGTTTCATAGGCACGGTGAA-FL |
gyrA-LC | LightCycler hybridization probe, reference gene, gyrA in GBS | R640-ACCTGAACGTCCCATCACCAAG-PH |
gyrA-R2 | LightCycler primer, reference gene, gyrA in GAS | 5′ CACTCCTTCACGGCTAGATT 3′ |
gyrA S | LightCycler primer, reference gene, gyrA in GAS | 5′ CTGGACCTGACTTTCCG 3′ |
gyrA-FL2 | LightCycler hybridization probe, reference gene, gyrA in GAS | ATTGACGCCATAGGGAAATTCAGTAACC-FL |
gyrA-LC2 | LightCycler hybridization probe, reference gene, gyrA in GAS | R640-CAATACGTTCCCGACCTGTTTGAGTTG-PH |
scpB-se | LightCycler primer, scpB | 5′ CAGCTGAGGTCACAATGCTAAC 3′ |
scpB-as | LightCycler primer, scpB | 5′ GCTCAAGCTATCAGAGATGCTGTC 3′ |
scpB-FL | LightCycler hybridization probe, scpB | GGTTGGCGTAAGCTAGTGCAGCA-FL |
scpB-LC | LightCycler hybridization probe, scpB | R640-TACCAAAGCTCATATTAATCACCTTAGCTCCC-PH |
lmb-fw | LightCycler primer, lmb | 5′ GCCTTGTGTGACTTCCATATCTT 3′ |
lmb-as | LightCycler primer, lmb | 5′ TGATGTGAGGATGATCCAATCAG 3′ |
lmb-FL | LightCycler hybridization probe, lmb | CGCGTCATAAATAGCTGCCACATCATTTACA-FL |
lmb-LC | LightCycler hybridization probe, lmb | R640-ACGGTTCAAAGGAATGAATGCCTGCA-PH |
GAS, group A streptococci.
FL, fluorescein; R640, LightCycler Red640 N-succinimide ester; PH, 3′ phosphate.