TABLE 2.
Characterization of influenza A viruses from diagnostic samples
| Sample no. | Virusa | M gene-specific rRT-PCR resultb | RT-PCR resultc | ArrayTube assay result |
|---|---|---|---|---|
| 1 | A/duck/Germany/R4-Lippe/08 (H1N1) | 26.64 | ++ | N1 |
| 2 | A/Anas platyrhynchos/Germany/R91/08 (H3N8) | 31.32 | + | N8 |
| 3 | A/Branta canadanensis/Germany/R107/08 (H11N9) | 34.06 | + | N9 |
| 4 | A/Cygnus olor/Germany/R108/08 (H4Nx) | 36.95 | + | N6 |
| 5 | A/Anser albifrons/Germany/R165/08 | 32.38 | + | N2 |
| 6 | A/Anser albifrons/Germany/R167/08 | 29.08 | ++ | N8 |
| 7 | A/Anser albifrons/Germany/R168/08 | 31.58 | + | N2 |
| 8 | A/Anser albifrons/Germany/R169/08 | 32.74 | + | N2 |
| 9 | A/Anas platyrhynchos/R234/08 (H1N1) | 29.91 | ++ | N1 |
| 10 | A/Anser anser/Germany/R239/08 (H9N2) | 33.96 | + | N2 |
| 11 | A/Anser anser/Germany/R240/08 (H9N2) | 32.34 | + | N2 |
| 12 | A/Anser anser/Germany/R248/08 (H9N2) | 22.33 | ++ | N2 |
| 13 | A/Struthio camelus/South Africa/R296/2/08 (H5N2) HPAIV | 26.35 | +/− | N2 |
| 14 | A/Struthio camelus/South Africa/R296/4/08 (H5N2) HPAIV | 18.98 | ++ | N2 |
| 15 | A/Struthio camelus/South Africa/R296/5/08 (H5N2) HPAIV | 25.86 | ++ | N2 |
| 16 | A/Struthio camelus/South Africa/R296/7/08 (H5N2) HPAIV | 30.50 | − | N2 |
| 17 | A/Callonetta leucophrys/Germany/R641/2/08 (H6N2) | 23.40 | ++ | N2 |
| 18 | A/Anas platyrhynchos/Germany/R665/2/08 (H5Nx) | 36.73 | − | N3 |
| 19 | A/Anas platyrhynchos/Germany/R695/08 (H5Nx) | 28.47 | + | N2 |
| 20 | A/Anas platyrhynchos/Germany/R701/08 (H5Nx) | 34.42 | − | N2 |
| 21 | A/Anas platyrhynchos/Germany/R726/08 (H5Nx) | 29.37 | + | N3 |
| 22 | A/Anas platyrhynchos/Germany/R734/1/08 (H5N3) | 24.23 | ++ | N3 |
| 23 | A/domestic duck/Germany/R786/08 (H5N1) HPAIV | 24.84 | ++ | N1 |
| 24 | A/domestic duck/Germany/R821/08 (H5N1) HPAIV | 36.91 | − | N1 |
| 25 | A/domestic duck/Germany/R836/08 (H5N1) HPAIV | 36.68 | − | N1 |
| 26 | A/domestic goose/Germany/R1110/08 (H6N2) | 30.95 | ++ | N2 |
| 27 | A/domestic goose/Germany/R1125/08 (H6N2) | 30.49 | ++ | N2 |
| 28 | A/mallard/Germany/R1140/2008 (H6N2) | 16.01 | +++ | N2 |
| 29 | A/mallard/Germany/R1142/2008 (H6N2) | 14.54 | +++ | N2 |
| 30 | A/Anser anser/Germany/R1416/08 (H2N9) | 24.31 | +++ | N9 |
| 31 | A/Anas platyrhynchos/Germany/R1419/08 | 30.67 | ++ | N9 |
| 32 | A/Anas platyrhynchos/Germany/R1423/08 (H5Nx) | 33.74 | + | N3 |
| 33 | A/Anas platyrhynchos/Germany/R1430/08 (H10Nx) | 29.02 | +++ | N9 |
| 34 | A/Anas platyrhynchos/Germany/R1433/08 | 33.08 | + | N9 |
| 35 | A/Anser anser/Germany/R1467/08 (H2N9) | 25.24 | +++ | N9 |
| 36 | A/Anas platyrhynchos/Germany/R1516/08 (H9N2) | 36.55 | − | N2 |
| 37 | A/Anas platyrhynchos/Germany/R1521/08 (H9N2) | 33.19 | + | N2 |
| 38 | A/Anas platyrhynchos/Germany/R1522/08 (H9N2) | 30.11 | + | N2 |
| 39 | A/Anas platyrhynchos/Germany/R1523/08 (H11N9) | 28.57 | ++ | N9 |
| 40 | A/Anser aegyptiacus/Germany/R1524/08 | 32.84 | ++ | N9 |
| 41 | A/Anas platyrhynchos/Germany/R1526/08 (H2N3) | 33.97 | + | N3 |
| 42 | A/wild bird/Germany/R1527/08 (H6N2) | 32.47 | + | N2 |
| 43 | A/Cygnus olor/Germany/R1541/08 (H7N3) | 34.55 | +/− | N3 |
| 44 | A/Anas platyrhynchos/Germany/R1594/08 (H11N1) | 28.22 | ++ | N1 |
| 45 | A/Anas platyrhynchos/Germany/R1597/08 (H11N1) | 28.01 | ++ | N1 |
| 46 | A/Anas platyrhynchos/Germany/R1599/08 (H9N2) | 28.65 | +++ | N8, N2 |
| 47 | A/Anas platyrhynchos/Germany/R1601/08 (H5N2) | 32.91 | + | N2 |
| 48 | A/Anas platyrhynchos/Germany/R1603/08 | 30.89 | ++ | N9 |
| 49 | A/Anas platyrhynchos/Germany/R1604/08 | 27.67 | ++ | N9 |
| 50 | A/Anas platyrhynchos/Germany/R1609/08 | 29.11 | ++ | N2 |
| 51 | A/Anser anser/Germany/R1625/08 (H6N2) | 29.73 | ++ | N2 |
| 52 | A/Anser anser/Germany/R1628/08 (H6N2) | 30.35 | ++ | N2 |
| 53 | A/Meleagris gallopavo/Germany/R1649-53/08 (H5N3) | 30.09 | +++ | N3 |
| 54 | A/domestic duck/Germany/R1769/08 (H6N2) | 33.60 | − | N2 |
| 55 | A/domestic duck/Germany/R1774/08 (H6N2) | 29.06 | +/− | N2 |
| 56 | A/domestic duck/Germany/R1779/08 (H6N2) | 32.92 | +/− | N2 |
| 57 | A/Meleagris gallopavo/Germany/R1914-6/08 (H5N3) | 25.84 | +++ | N3 |
| 58 | A/Meleagris gallopavo/Germany/R2040/08 | 38.02 | − | —d |
| 59 | A/Meleagris gallopavo/Germany/R2045/08 (H5N3) | 32.56 | + | N3 |
| 60 | A/Anas platyrhynchos/Germany/R2316/08 | 32.20 | + | N1 |
| 61 | A/Anas platyrhynchos/Germany/R2319/08 | 31.70 | + | N1 |
| 62 | A/Cygnus cygnus/Germany/R2324/08 (H6N5) | 34.22 | + | N5 |
| 63 | A/Cygnus cygnus/Germany/R2325/08 (H6N5) | 33.74 | + | N5 |
| 64 | A/duck/Germany/R2331/0 8 (H5N3) | 27.86 | ++ | N3 |
| 65 | A/duck/Germany/R2342/0 8 (H5N3) | 31.15 | ++ | N3 |
| 66 | A/Meleagris gallopavo/Germany/R2379/08 (H5N3) | 26.88 | ++ | N3 |
| 67 | A/Anser albifrons/R2/09 (H6N8) | 27.10 | ++ | N8 |
| 68 | A/Anser albifrons/R3/09 (H6N8) | 31.99 | + | N8 |
| 69 | A/Meleagris gallopavo/Germany/R23/09 (H3N2) | 34.14 | − | N2 |
| 70 | A/Meleagris gallopavo/Germany/R24/09 (H3N2) | 35.48 | − | N2 |
| 71 | A/Larus canus/Germany/R127/09 | 27.08 | − | N6 |
| 72 | A/Larus argentatus/Germany/R128/09 | 29.24 | − | N6 |
| 73 | A/Larus argentatus/Germany/R129/09 | 29.42 | +/− | N6 |
| 74 | A/Anas platyrhynchos/Germany/R131/09 | 21.21 | ++ | N2 |
| 75 | A/Anas platyrhynchos/Germany/R132/09 | 36.67 | − | N2 |
| 76 | A/Anas platyrhynchos/Germany/R133/09 | 32.28 | +/− | N2 |
a
Subtyping was not successful for all samples. NA subtypes were determined by subtype-specific RT-PCRs followed by sequencing (4) and/or by sequencing of the entire NA gene (10).
b
CT (dR) value for M gene-specific rRT-PCR (15).
c
+++ to +, different intensities of bands by agarose gel electrophoresis; +/−, weak band; −, no band visible.
d
For sample no. 58, no ArrayTube assay result could be achieved.