TABLE 4.
Specificity of the FluPlex for all HA and NA subtypes of influenza A and influenza B virus
| Virus | Target(s)a | No. of copies/ reaction | Resultb for:
|
||||
|---|---|---|---|---|---|---|---|
| HA/NA-specific probe | All nonspecific HA probes | All nonspecific NA probes | Influenza virus A matrix gene probe | Influenza virus B NS gene probe | |||
| A/WI/629-9/08 (H1N1) | H1/N1/FA virus | 2.9 × 106 | 4.0/4.0 | NA | NA | 4.0 | NA |
| A/Singapore/1/57 (H2N2) | H2/N2/FA virus | 1.5 × 104 | 4.0/4.0 | NA | NA | 4.0 | NA |
| A/WI/629-2/08 (H3N2) | H3/N2/FA virus | 1.5 × 104 | 4.0/4.0 | NA | NA | 4.0 | NA |
| A/Anhui/01/2005 (H5N1)-PR8-IBCDC-RG5 | H5/N1/FA virus | 2.2 × 107 | 4.0/4.0 | NA | NA | 4.0 | NA |
| A/Chicken/NJ/15086-3/94 (H7N3) | H7/N3/FA virus | 1.1 × 106 | 1.7/NA | NA | NA | 4.0 | NA |
| A/Chicken/NJ/12220/97 (H9N2) | H9/N2/FA virus | 1.8 × 106 | 4.0/4.0 | NA | NA | 4.0 | NA |
| A/GWT/LA/169GW/88 (H10N7) | N7/FA virus | 5.2 × 106 | NA/3.3 | 0.88 (H3)c | NA | 4.0 | NA |
| B/WI/629-1/2008 | NS gene | 1.6 × 102 | NA | NA | NA | NA | 1.9 |
| A/Mallard/OH/338/86 (H4N8) | FA virus | 3.8 × 106 | NA | NA | NA | 4.0 | NA |
| A/Chicken/CA/431/00 (H6N2) | N2/FA virus | 1.4 × 106 | N/4.0 | NA | NA | 4.0 | NA |
| A/Blue-Winged Teal/LA/B194/86 (H8N4) | FA virus | 6.9 × 106 | NA | NA | NA | 4.0 | NA |
| A/Chicken/NJ/15902-9/96 (H11N9) | FA virus | 1.6 × 105 | NA | NA | NA | 4.0 | NA |
| A/Duck/LA/188D/87 (H12N5) | FA virus | 2.3 × 107 | NA | NA | NA | 4.0 | NA |
| A/Gull/MD/704/77 (H13N6) | FA virus | 6.8 × 106 | NA | NA | NA | 4.0 | NA |
| A/Mallard/GurjevRussia/262/82 (H14N5) | FA virus | 2.2 × 105 | NA | NA | NA | 2.2 | NA |
| A/Shearwater/Australia/2576/79 (H15N9) | FA virus | 3.2 × 106 | NA | NA | NA | 4.0 | NA |
| A/Shorebird/DE/172/2006 (H16N3) | FA virus | 1.6 × 102d | NA | NA | NA | 3.6 | NA |
a
FA virus, influenza A virus.
b
Results are reported as the absorbance at 450 nm (the range is 0 to 4.0). NA, not applicable (no cross-reaction was detected for the specified probes).
c
Cross-reactivity was seen only at very high concentrations of H10. H3N7 has not been found in humans; therefore, positive detection of this combination or other unusual combinations would call for other tests for further verification.
d
The quantitation for H16N3 is expressed as TCID50 per reaction.