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Real-time RT-PCR results for serial dilutions of RNAs of BTV-1, -6, and -8 with the appropriate primer-probe mixtures. For BTV-1 and BTV-6, the alternative mixtures produced similar results (BTV1-VP2-Mix2-FAM, y = −3.4471x + 17.383 [R2 = 0.9983]; BTV6-VP2-Mix2-FAM, y = −3.2771x + 8.5275 [R2 = 0.999]; data not shown).
