TABLE 1.

Primers and probes used in this study

Oligonucleotide (product size [bp]) and type	Name	Sequence
BTV1-VP2-Mix1-FAM (123)
Forward primer	BTV1-VP2-186F	5′ CGG ACC GCA TTA TGG TAT AAC C 3′
Reverse primer	BTV1-VP2-308R	5′ ACT CTT GTG TCT CGT ACT TTC AAC 3′
Probe	BTV1-VP2-203FAM	5′ ACC GCC CGT CTT TCA TCG TAA CCC 3′
BTV1-VP2-Mix2-FAM (120)
Forward primer	BTV1-VP2-2407F	5′ CCT CAA AGG CGA TTC GAT TTA GC 3′
Reverse primer	BTV1-VP2-2526R	5′ TCA CGA CGT TGT AGT TGA CTC C 3′
Probe	BTV1-VP2-2438FAM	5′ TGA AGC GCA GCC CAA GAT TGC ACG 3′
BTV6-VP2-Mix1-FAM (97)
Forward primer	BTV6-VP2-785F	5′ GAT ACG TGA TGC GTG GAT TG 3′
Reverse primer	BTV6-VP2-881R	5′ TAC CAC CTT CCT TCC GAC AC 3′
Probe	BTV6-VP2-817FAM	5′ ATC CGA GGC ATA TTC GCT CGC TGG 3′
BTV6-VP2-Mix2-FAM (89)
Forward primer	BTV6-VP2-1056F	5′ TAT AAT GGC AGA ATA TGG TGG AC 3′
Reverse primer	BTV6-VP2-1144R	5′ CAG TAA ACA TCG CCC AAC CT 3′
Probe	BTV6-VP2-1081FAM	5′ ATC CGT ACC CTT GCT TGC GTG GAG 3′
BTV8-VP2-Mix1-FAM (86)
Forward primer	BTV8-VP2-1604F	5′ GTT ACG CAT TAC CGA GGT TGT G 3′
Reverse primer	BTV8-VP2-1689R	5′ GAT CAT GTG TGA ACG CCT TCG 3′
Probe	BTV8-VP2-1631FAM	5′ AAC GGC TCA CAC CGA CGA TCC AGC 3′