TABLE 1.
C. botulinum type B strains and sequences used in this studya
| Strain identifier | BoNT/B subtypeb | GenBank accession no. | Origin (country, yr) | Reference or source |
|---|---|---|---|---|
| Bacterial strain | ||||
| 111 | B2 | AB302854, AB084152 | Infant botulism (Japan, 1995) | 15 |
| Osaka05 | Osaka05 | AB302852 | Infant botulism (Japan, 2005) | In this study |
| Osaka06 | B2 | AB302853 | Infant botulism (Japan, 2006) | In this study |
| 89E00061-2 | B1 | AB302861 | Infant botulism (United States, 1989) | In this study |
| 89E00067-4 | B1 | AB302862 | Infant botulism (United States, 1989) | In this study |
| 89E00123-1 | B1 | AB302963 | Infant botulism (United States, 1989) | In this study |
| 90E00001-3 | B1 | AB302864 | Infant botulism (United States, 1990) | In this study |
| 3129-2-77 | B1 | AB302865 | Infant botulism (United States, 1990) | In this study |
| Okra | B1 | AB232927 | Food borne (unknown) | 23 |
| 326 | B1 | AB302858 | Pork meat (Japan) | In this study |
| 407 | B1 | AB302856 | Pork meat (Japan) | In this study |
| Ginger | B2 | AB302857 | Ginger (Japan) | In this study |
| 7H215S | B2 | AB302860 | Honey (Japan) | In this study |
| 9B | B1 | AB302859 | Stocked strain (United States) | In this study |
| 67B | B1 | AB302855 | Stocked strain (United States) | In this study |
| GenBank | ||||
| CDC1758 | B1 | EF033127 | Unknown | 13 |
| Danish | B1 | M81186 | Unknown | 41 |
| Hall6517(B) | B1 | EF028399 | Unknown | 13 |
| CDC1656 | B1 | EF028396 | Unknown | 13 |
| Prevot25 NCASE | B2 | EF033129 | Unknown | 13 |
| 213B | B2 | EF028395 | Unknown | 13 |
| Smith L-590 | B2 | EF028398 | Unknown | 13 |
| Prevot59 | B2 | EF033128 | Unknown | 13 |
| CDC1828 | B2 | EF051571 | Unknown | 13 |
| CDC6291 | B2 | EF028401 | Unknown | 13 |
| Korean soil 1 | B2 | DQ417353 | Korean soil | GenBank |
| Korean soil 2 | B2 | DQ417354 | Korean soil | GenBank |
| CDC795 | B3 | EF028400 | Unknown | 13 |
| CDC593 | Bivalent, A(B)c | AF300466 | Dog feces (United States, 1976) | 19 |
| CDC1436 | Bivalent, ABd | AF295926 | Stool sample (United States, 1977) | 19 |
| 657Ba | Bivalent, Bad | EF033130 | Unknown | 13, 36 |
| CDC588 | Bivalent, Abd | AF300465 | Food borne (United States, 1976) | 19 |
| CDC3281 | Bivalent, Bfd | Y13630 | Infant botulism (United States, 1980) | 33 |
| ATCC 17844 | Nonproteolytic | EF028394 | Unknown | 13 |
| Eklund17B (B257) | Nonproteolytic | EF051570 | Unknown | 13 |
| 10068 | Nonproteolytic | EF028402 | Unknown | 13 |
| Eklund17B | Nonproteolytic | X71343 | Unknown | 14 |
a
The subtypes and GenBank accession numbers for strains determined in this study are indicated in boldface.
b
Subtyped by Hill et al. (13); the other strains were subtyped in this study.
c
BoNT/A producing and unexpressed boNT/B gene possessing.
d
Dual toxin-producing strains; the major toxin type is indicated in uppercase letters and the minor type is indicated in lowercase letters.