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. 2009 Jun 10;83(17):8327–8339. doi: 10.1128/JVI.00586-09

FIG. 3.

FIG. 3.

Analysis of the effects of the mutations stabilizing the 5′-terminal stem of the VEE/SINV genome on virus viability and replication. (A) Computer-predicted secondary structures of the 5′ termini in the genomes of VEE/SINV variants having the mutations that increased the stability of the RNA stem. Open circles indicate positions of the mutations. RNA transfections were performed as described in Materials and Methods. RNA infectivities in the infectious center assay and titers of the viruses at 48 h posttransfection are indicated. PEP, postelectroporation. (B) Nucleotide sequences of the heterologous 5′ ends in the genomes of (C19C20)VEE/SINV and (C17C19C20)VEE/SINV pseudorevertants present in the plaques, which were randomly selected in the infectious center assay. Heterologous sequences are indicated by boldface type. Lowercase type indicates mutations introduced into (G3)VEE/SINV to generate (C19C20)VEE/SINV and (C17C19C20)VEE/SINV. The number of cDNA clones encoding a particular sequence is indicated. Orig., origin.