FIG. 6.

NS2 suppressors in the WT background. (A) Replication of Jc1/GLuc2A containing the indicated second-site changes at 8, 24, 48, 72, and 96 h posttransfection. Values represent RLU averages from at least three independent transfections; error bars represent the standard deviation of the mean. (B) The medium collected in panel A was used to infect naive cells and calculate relative infectivities as in Fig. 1. Values represent average relative infectivity measurements from at least three independent transfections; error bars represent the standard deviation of the mean. (C) NS2 mutations and suppressors function in the absence of the GLuc2A insert. Infectious virus production was measured for Jc1 NS2 mutants, which lack the GLuc2A insert, by limiting dilution of the cell culture medium harvested at 48 h posttransfection. Values represent TCID₅₀ calculated from three independent transfections; error bars represent the standard error of the mean.