TABLE 1.

Site-directed mutagenesis of NS2

NS2 mutation	Jc1 codon	H77 codon	Primer sequence (5′-3′)a
G10A	823	819	TCTGTGCATGGCCAGATAGCCGCGGCTCTGCTGGTAATG
P24A	837	833	CACTCTCTTTACTCTCACCGCCGGGTATAAGACCCT
K27A	840	836	CTCTCACCCCCGGGTATGCCACCCTCCTCGGCCAGTGT
W35A	848	844	TCCTCGGCCAGTGTCTGGCCTGGTTGTGCTATCTCCTG
W36A	849	845	TCCTCGGCCAGTGTCTGTGGGCCTTGTGCTATCTCCTG
Y39A	852	848	TGTCTGTGGTGGTTGTGCGCCCTCCTGACCCTGGGGGAA
E45A	858	854	TCTCCTGACCCTGGGGGCCGCCATGATTCAGGAGT
E45K	858	854	TCTCCTGACCCTGGGGAAGGCCATGATTCAGGAGT
P53A	866	862	TGATTCAGGAGTGGGTAGCCCCCATGCAGGTGCG
R58A	871	867	TACCACCCATGCAGGTGGCCGGCGGCCGCGATGGCAT
R61A	874	870	ATGCAGGTGCGCGGCGGCGCCGATGGCATCGCGTGG
D62A	875	871	AGGTGCGCGGCGGCCGCGCCGGCATCGCGTGGGCCGT
P73A	886	882	CCGTCACTATATTCTGCGCCGGTGTGGTGTTTGACATT
F77A	890	886	TCTGCCCGGGTGTGGTGGCCGACATTACCAAATGGCTT
D78A	891	887	TGCCCGGGTGTGGTGTTTGCCATTACCAAATGGCTTT
D78K	891	887	TGCCCGGGTGTGGTGTTTAAGATTACCAAATGGCTTT
K81A	894	890	TGGTGTTTGACATTACCGCCTGGCTTTTGGCGTTGCT
K81E	894	890	TGGTGTTTGACATTACCGAATGGCTTTTGGCGTTGCT
P89A	902	898	CTTTTGGCGTTGCTTGGGGCCGCTTACCTCTTAAGGGCCGCTT
R111A	924	920	GAGCTCACGCTCTGATAGCCGTATGCGCTTTGGTGAA
Y124A	937	933	AAGCAGCTCGCGGGGGGTAGGGCCGTTCAGGTGGCGCTATT
R156A	969	965	ACTGGGCCGCTAGCGGCCTGGCCGACTTAGCGGTCGCCGT
D157A	970	966	ACTGGGCCGCTAGCGGCCTGCGCGCCTTAGCGGTCGCCGT
V160A	973	969	TGACACATGTGCCGTACGCCGTCAGAGCTCACGCTCTG
S168A	981	977	TGGAACCCATCATCTTCGCCCCGATGGAGAAGAAGGT
S168D	981	977	TGGAACCCATCATCTTCGACCCGATGGAGAAGAAGGT
S168E	981	977	TGGAACCCATCATCTTCGAGCCGATGGAGAAGAAGGT
E171A	984	980	TCATCTTCAGTCCGATGGCCAAGAAGGTCATCGTCTG
K173A	986	982	TCAGTCCGATGGAGAAGGCCGTCATCGTCTGGGGAGC
D180A	993	989	TCATCGTCTGGGGAGCGGCCACGGCTGCATGTGGGGA
D181A	994	990	ATCGTCTGGGGAGCGGAGGCCGCTGCATGTGGGGACA
R196A	1009	1005	ACTTCCCGTGTCCGCCGCCCTCGGCCAGGAGATCCTC

^aForward primers are shown; complementary reverse primers were also used in site-directed mutagenesis. Mutation sites are underlined.