TABLE 2.
Primers used to amplify the HCV coding region for sequencing
| Amplicon | Orientation | Sequence (5′-3′) |
|---|---|---|
| 1 | Forward | TCCCGGGAGAGCCATAGTGGT |
| Reverse | AGCCATCCTGCCCAGCCGAGTC | |
| 2 | Forward | CCAGCCCATCCCTAAAGATCGG |
| Reverse | CACGGCCACATTCGGTGAGA | |
| 3 | Forward | CGGCTACATGGTGACTAACGAC |
| Reverse | GCCAACAGAAGGATGACAACGA | |
| 4 | Forward | GCTCTGCTCTGCCCTCTACGTG |
| Reverse | CGGACATGCGTTCGGGACA | |
| 5 | Forward | TCCTTGCACACCGGCTTTA |
| Reverse | GCCGCATTTGAGGTAAGTGGTA | |
| 6 | Forward | TTCAACGCCAGCACGGACCTGT |
| Reverse | TCCATAAGCAGGCGCAAAC | |
| 7 | Forward | CAATTCATGTATGGCCTATCA |
| Reverse | CTCTTTGTCGCCTTCGT | |
| 8 | Forward | CACCAGGGGCTGTCTGATCT |
| Reverse | CGCCACCTGAACATACCTACC | |
| 9 | Forward | AGGGCCGCTTTGACACA |
| Reverse | TCGACGGCTCCACACTTGCAC | |
| 10 | Forward | TGCTGAGGGGGACTTGGTAG |
| Reverse | GCCGTAGCCAGCACAGTTAGTCTGA | |
| 11 | Forward | CGATGAATGCCACGCTGTGGATGCT |
| Reverse | GGGAGAGGAAGTGGGCGTCTA | |
| 12 | Forward | CCGTCAGGCTTAGAGCGTATTTCA |
| Reverse | AGTGTTGACAATCCTGCGAGGTATT | |
| 13 | Forward | AGCAGGCCCAGGACATACAAC |
| Reverse | CAGCTTGGGGAACAATTTAGAGGTC | |
| 14 | Forward | GTGTGGGACTGGGTTTGCACCATCT |
| Reverse | GGCTCGAGAAAGTCCAGAACGG | |
| 15 | Forward | TGACGTGGACATGGTCGATGCCAAC |
| Reverse | CCGCTAGCTTGATGTCCTTTAAGAC | |
| 16 | Forward | TCACAGAGGGCTAAAAAGGTAACT |
| Reverse | TCCCCTGGCTTTCTGAGATGACTAC | |
| 17 | Forward | CCATGTTCAACAGCAAGGGTCAAAC |
| Reverse | CGACGGTGAACCAACTGGATAAGTC | |
| 18 | Forward | GAGAGGTTACACGGGCTTGAC |
| Reverse | GGACCTTTCACAGCTAGCCGTGACT |